Biofilm on Chest Drains After Thoracic Surgery: Clinical Impact and Antibiotic Resistance
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18+
SponsorCaner İşevi, MD
About this trial
This prospective observational study aims to investigate the frequency and clinical significance of biofilm formation on thoracic chest drains used after thoracic surgery. Biofilms are structured bacterial communities that adhere to surfaces and can promote infection and antibiotic resistance. In this study, small samples from the tips of chest drains will be collected under sterile conditions at the time of drain removal and analyzed in the microbiology laboratory.
The main objectives are to evaluate the relationship between the duration of chest drain placement and the presence of biofilm, and to identify the antibiotic resistance profiles of microorganisms isolated from these biofilms. Secondary outcomes include the association between biofilm formation, postoperative infections (such as empyema or wound infection), and the length of hospital stay.
This study will be conducted at Ondokuz Mayıs University Faculty of Medicine, Department of Thoracic Surgery, in collaboration with the Department of Microbiology. The findings are expected to contribute to better understanding of optimal drain management and infection prevention strategies after thoracic surgery.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Age 18 years and older
Undergoing elective thoracic surgery requiring chest drain placement
Able and willing to provide written informed consent
Disqualifiers
Presence of active infection prior to surgery
Emergency thoracic surgery
Immunosuppressed patients (e.g., neutropenia, advanced malignancy, organ transplantation)
Patients receiving systemic antibiotic therapy at the time of drain removal
Trial population
Adult patients (≥18 years old) who undergo elective thoracic surgery at Ondokuz Mayıs University Faculty of Medicine and require postoperative chest drain placement. Participants are prospectively enrolled and observed until drain removal. Drain tip samples are collected for microbiological and biofilm analysis.
Trial design
Design model
Cohort
Time perspective
Prospective
Treatments tested in this trial
Chest Drain Tip Sampling
Other interventionNo active intervention; routine chest drain removal followed by microbiological and biofilm analysis of drain tip samples.
Treatment groups
180 Participants
are divided into 3 treatment groupsGroup A: Group 1 -Short Drain Duration (≤2 Days)1 intervention
Group B: Group 2 - Moderate Drain Duration (3-4 Days)1 intervention
Group C: Group 3 - Long Drain Duration (≥5 Days)1 intervention
Trial outcomes
Primary outcomes
1
Presence and Intensity of Biofilm Formation on Chest Drain Tips
Biofilm formation on the inner surface of chest drain tips will be evaluated using a microplate crystal violet staining method. The optical density (OD) at 492 nm will be measured, and biofilm intensity will be classified according to the Christensen and Chusri scale (non, weak, moderate, strong). The proportion of patients with biofilm-positive drain tips will be recorded and compared across drain duration groups.
Time frame
At the time of chest drain removal (postoperative days 1-7)Sponsors and contacts
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Caner İşevi, MD
Lead sponsor
Ondokuz Mayıs University
Sponsor institution
Source ClinicalTrials.gov