Comparison of Aquaporin -4, -5, -9 and IL-8 Levels in GCF, Dentin Fluid and Pulp Samples
Trial statusNot yet recruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age18-64
SponsorKırıkkale University
About this trial
This study aimed to compare the changes in AQP -4, -5, -9, and IL-8 levels in pulp tissue, GCF, and dentin fluid samples routinely obtained during the treatment of healthy and symptomatic teeth diagnosed with irreversible pulpitis and investigate whether there is a correlation between them.
A total of 70 patients aged 18-64 years with healthy (Group 1) and symptomatic irreversible pulpitis (Group 2) diagnosed at Kırıkkale University, Faculty of Dentistry, Department of Endodontics who will undergo routine root canal treatment will be included in the study, with a minimum of 35 participants for each group. Before starting treatment, DOS samples will be taken from healthy, symptomatic, irreversible pulpitis-diagnosed teeth and teeth contralateral to these teeth. A dentin fluid sample will be taken by holding the membrane on the dentin surface, and the pulp tissue will be removed and transferred to Eppendorf tubes. The treatment process will be completed by applying routine root canal procedures to the teeth. The samples' Aquaporin -4, -5, -9, and IL-8 levels will be analyzed by specific enzyme-linked immunosorbent assay (ELISA).
Eligibility criteria
This trial accepts healthy volunteersQualifiers
Patients aged 18 to 64 years.
Patients without any known systemic disease.
Patients requiring root canal treatment for prosthetic purposes (Healthy pulp group).
Patients with third molars (wisdom teeth) indicated for extraction due to orthodontic or other clinical reasons (Healthy pulp group).
Disqualifiers
Patients with any known systemic disease.
Pregnant women or those suspected of being pregnant.
Patients who have used antibiotics, anti-inflammatory drugs, or antidepressants within the past 4 weeks.
Patients with significant dental plaque or calculus, gingival redness or bleeding, severe gingivitis, generalized periodontitis, or periodontal pockets deeper than 4 mm.
Trial population
This study will include patients aged 18-64 years who present to the Department of Endodontics, Faculty of Dentistry, Kırıkkale University, with an indication for root canal therapy and are diagnosed with either a healthy pulp status (Group 1) or symptomatic irreversible pulpitis (Group 2).
Trial design
Design model
Other
Time perspective
Cross-sectional
Treatments tested in this trial
Not listed
Trial groups
70 Participants
are grouped into 2 trial groupsGroup A: healthy pulp group
Group B: irreversible pulp group
Trial outcomes
Primary outcomes
1
Aquaporin-4, Aquaporin-5 and Aquaporin-9 expression levels in pulp tissue in dentin fluid and GCF samples
Levels of IL-8, AQP-4, AQP-5 and AQP-9 will be measured in dentin fluid, GCF and pulp tissue samples obtained during routine root canal treatment. The analysis will be performed using ELISA. The aim is to compare the expression levels between healthy individuals and patients with irreversible pulpitis.
Time frame
Immediately after the procedureSecondary outcomes
1
Correlation analysis between Aquaporin (AQP-4, AQP-5, AQP-9) and IL-8 levels in pulp tissue, dentin fluid, and GCF samples
The study will assess the correlation between Aquaporin isoforms (AQP-4, AQP-5, and AQP-9) and IL-8 concentrations within and between two groups: healthy individuals and patients diagnosed with irreversible pulpitis. Correlation analyses will be conducted separately for each group, as well as comparatively across groups, using appropriate statistical methods to explore intra- and intergroup relationships among biomarkers in pulp tissue, dentin fluid, and GCF samples.
Time frame
Immediately after the procedureLocations
Sponsors and contacts
Click on the lead sponsor to view all of their trials.
Source ClinicalTrials.gov
This trial is not recruiting at the moment. You can still explore other options: