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Comparison of the Effects of Aerobic-Anaerobic Exercises on Hormonal and Immune Biomarkers

ConditionsActivity, MotorBiomarkersExerciseImmunity
Trial statusNot yet recruiting
Trial phaseNot applicable
Trial typeInterventional
Biological sexAll
Age18-25
SponsorSakarya Applied Sciences University

About this trial

This study aims to compare the effects of aerobic and anaerobic exercise on hormonal, immunological, and metabolic biomarkers in young individuals using blood and saliva samples. It will also assess participants' physical activity levels, depression levels, and general lifestyle habits to explore their relationship with biomarker profiles. Biomarkers such as testosterone, progesterone, cortisol, IgA, alpha-amylase, insulin, lactate, and various inflammatory cytokines will be measured using ELISA. The study seeks to evaluate the physiological and psychosocial effects of different types of exercise in a holistic manner.

Eligibility criteria

This trial accepts healthy volunteers

Qualifiers

Being a university student,

Being between the ages of 18 and 25.

Disqualifiers

Having orthopedic problems that prevent exercise,

Having cognitive or mental health problems that prevent participation in exercise,

Having chronic systemic diseases such as cardiac, pulmonary, or nephrological.

Trial design

Design model

Parallel

Treatments tested in this trial

  • Treadmill Exercise

    Device

    Participants in this group will perform an aerobic exercise using the Bruce Protocol on a treadmill. The test involves walking or running with gradually increasing speed and incline in predefined stages to assess cardiovascular endurance. Heart rate, blood pressure, and fatigue levels will be recorded at each stage. The session will conclude with a low-intensity cool-down phase.

  • Bicycle Exercise

    Device

    The Wingate Anaerobic Test will be conducted using an ergometer bike. After a standardized warm-up, participants will pedal all-out for 30 seconds against a preset resistance, aiming to evaluate their anaerobic performance. Physiological parameters including power output, heart rate, and oxygen saturation will be monitored throughout the test.

Treatment groups

90 Participants
are divided into 3 treatment groups
Group A: Group I - Control Group:No intervention 0 interventions
Group B: Group II - Treadmill Exercise (Bruce Protocol):Experimental treatment 1 intervention
Group C: Group III - Bicycle Exercise (Wingate Anaerobic Test):Experimental treatment 1 intervention

Trial outcomes

Primary outcomes

1

Change in Salivary Testosterone Level

Measurement of testosterone concentration in saliva pre- and post-exercise.

Time frame
Time Frame: Baseline and immediately post-exercise Unit of Measure: pg/mL
2

Change in Salivary Cortisol Level

Measurement of cortisol concentration in saliva before and after aerobic and anaerobic exercise protocols.

Time frame
Time Frame: Baseline (pre-exercise) and immediately post-exercise (within 1 hour) Unit of Measure: ng/mL
3

Change in Salivary Progesterone Level

Salivary progesterone levels before and after exercise.

Time frame
Time Frame: Baseline and immediately post-exercise Unit of Measure: pg/mL
4

Change in Salivary Alpha-Amylase Activity

Enzymatic activity of salivary alpha-amylase as a stress marker.

Time frame
Time Frame: Baseline and post-exercise Unit of Measure: U/mL

Secondary outcomes

1

Change in Depression Score (Beck Depression Inventory-II)

Assessment of participants' depressive symptoms using the validated Beck Depression Inventory-II (BDI-II).

Time frame
Time Frame: Baseline (pre-intervention) and post-intervention (within 1 hour after final session) Unit of Measure: Score (range: 0-63) Method of Measurement: Self-reported questionnaire
2

Change in Physical Activity Level (IPAQ-Short Form)

Evaluation of participants' physical activity levels using the International Physical Activity Questionnaire - Short Form (IPAQ-SF), which estimates physical activity over the last 7 days in MET-minutes/week.

Time frame
Time Frame: Baseline and post-intervention Unit of Measure: MET-minutes/week

Other outcomes

Locations

This trial has no locations.

Sponsors and contacts

Click on the lead sponsor to view all of their trials.

Sakarya Applied Sciences University

Lead sponsor

Source ClinicalTrials.gov

This trial is not recruiting at the moment. You can still explore other options: