Detection of Disease Marker Factors in Cyst Fluid
ConditionBiomarkers
Trial statusRecruiting
Trial phaseNot listed
Trial typeObservational
Biological sexAll
Age12-80
SponsorHarran University
About this trial
The purpose of this observational study is to examine the cyst fluid obtained after a puncture procedure performed for cyst diagnosis in participants with cystic lesions on clinical and radiological examination.The main question it aims to answer is:
\- What is the exact mechanism of formation of odontogenic cysts?
Biomarkers in the cyst fluid obtained after a puncture procedure performed as part of the diagnosis and treatment process of odontogenic cysts will be examined. The aim of this study is to gain insight into cyst pathology by examining the levels of biomarkers in odontogenic cyst fluid.
Eligibility criteria
This trial does not accept healthy volunteersQualifiers
Individuals diagnosed with cysts
Individuals who are not pregnant or breastfeeding
Individuals who agree to sign the informed consent form
Disqualifiers
Systemically ill individuals who are contraindicated for surgery.
Individuals who do not wish to sign the informed consent form.
Individuals who are pregnant, breastfeeding, or using oral contraceptives will be excluded.
Trial population
The study group will consist of individuals aged 12-80 with cystic lesions who agree to participate in the study themselves and with their families (if children), and who are not pregnant or breastfeeding.
Trial design
Design model
Other
Time perspective
Prospective
Treatments tested in this trial
cyst fluid examination
Other interventionParticipants will not undergo any procedures outside of the routine for the study. The cyst fluid obtained during the puncture will be collected in a tube and centrifuged at 3000 rpm for 20 minutes at 4 degrees Celsius. The Eppendorf tubes will be stored at -80 °C until the clear liquid remaining above is taken and examined, without removing the particles that have settled at the bottom of the tube. The biochemical parameters of the cyst fluid obtained from the study participants will be analyzed using the ELISA method. MMP-8, MMP-9, RANKL, and MIP1alpha will be analyzed using commercially available ELISA kits, following the manufacturer's instructions.
Treatment groups
25 Participants
are divided into 1 treatment groupGroup A: individuals with odontogenic cysts1 intervention
Trial outcomes
Primary outcomes
1
MMP-8 concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MMP-8 concentration level will be measured.
Time frame
through study completion, an average of 6 months2
MMP-9 concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MMP-9 concentration level will be measured.
Time frame
through study completion, an average of 6 months3
RANKL concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the RANKL concentration level will be measured.
Time frame
through study completion, an average of 6 months4
MIP-1alpha concentration level in cyst fluid
Cyst fluids aspirated during the puncture will be centrifuged using a centrifuge and stored at -80 degrees Celsius in Eppendorf tubes until examined. After all samples are collected, the MIP-1alpha concentration level will be measured.
Time frame
through study completion, an average of 6 monthsSecondary outcomes
1
Correlation between the type of cyst and the measured markers
After measuring the concentration levels of the MMP-8, MMP-9, RANKL, and MIP-1alpha biomarkers in the cyst fluid, the results will be compared with the subsequent biopsy results to examine their correlation with the type and origin of the cyst.
Time frame
through study completion, an average of 6 monthsSponsors and contacts
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Source ClinicalTrials.gov