Acne vulgaris is a common chronic inflammatory skin disorder that affects adolescents and young adults. Recent research has shown that lipid mediators, including ceramides and sphingolipids, play an important role in maintaining skin barrier function and inflammation.
This study aims to evaluate the serum levels of ceramide C16, ceramide C24, and sphingosine-1-phosphate (S1P) in patients with acne vulgaris and compare them to healthy controls. The goal is to explore their possible role in the pathogenesis and severity of acne. Blood samples will be collected from participants, and quantitative analysis will be performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
For Case Group
Both males and females aged 15-40 years.
Mild, Moderate, severe and very severe cases will be included. 5
Presence of any dermatological condition other than acne vulgaris (for cases).
Use of systemic or topical treatment such as isotretinoin or antibiotics within the last 3 months.
Pregnancy or lactation.
History of lipid-lowering therapy or supplements affecting ceramide or sphingolipid metabolism.
Study Population: A. Inclusion Criteria: For Case Group This group will consist of (40) patients : * Both males and females aged 15-40 years. * Mild, Moderate, severe and very severe cases will be included. 5 For Control Group: • This group will consist of age- and sex-matched (40) healthy volunteers. B. Exclusion Criteria (for both groups): * Presence of any dermatological condition other than acne vulgaris (for cases). * Use of systemic or topical treatment such as isotretinoin or antibiotics within the last 3 months. * Pregnancy or lactation. * History of lipid-lowering therapy or supplements affecting ceramide or sphingolipid metabolism
Case-control
Cross-sectional
Blood sample collection for biochemical analysis of serum ceramides (C16, C24) and sphingosine-1-phosphate (S1P).
Other interventionVenous blood samples (5 mL) will be collected from all participants under aseptic conditions. Serum will be separated and stored at -80°C until analysis. Quantitative estimation of ceramide C16, ceramide C24, and sphingosine-1-phosphate (S1P) will be performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). No therapeutic intervention will be given.
Serum levels of Ceramide C16, Ceramide C24, and Sphingosine-1-Phosphate (S1P)
Quantitative assessment of serum concentrations of Ceramide C16, Ceramide C24, and Sphingosine-1-Phosphate (S1P) in acne vulgaris patients and healthy controls using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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