The purpose of this study is to determine how anabolic sensitivity, in response to the ingestion of a liquid protein meal, differs across the lifespan, between biological sexes, and with varying physical activity levels.
Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone)
Chronological age for Healthy Young (age: 18-35 years) or Older (age: 60-80 years) adults
Chronological age between 8 to 16 years, with maturity offset < -1 year from age of Peak Height Velocity (aPHV) for children and between -0.5 to +1.5 years from aPHV in adolescent participants
BMI between normal to overweight (18.5 - 29.9 kg/m2)
Regular use of nonsteroidal anti-inflammatory dugs (with the exception of daily low-dose aspirin)
use of anticoagulants
use of a walker, cane, or assistive walking device
infectious or gastrointestinal disease
Convenience sample of individuals who qualify for the corresponding cohorts based on inclusion/exclusion criteria stated.
Cohort
Cross-sectional
Not listed
Amino acid oxidation and net protein balance
Amino acid oxidation and net protein balance assessed by oral 1-13C Leucine tracer. Net protein balance is derived from the difference between amino acid intake (known) and total amino acid oxidation over the 6h post-prandial measurement period.
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